Coding
spilk 7.0.

Part:BBa_M36500

Designed by: Dominique Dabija, Christopher Jackson, Debha Amatya   Group: Stanford BIOE44 - S11   (2011-12-03)


Spider Silk Hexamer

This is a spider silk hexamer protein. The monomer amino acid sequence was taken from Xia et al. and codon optimized for expression in E. coli. Repeats above 10 base pairs were also minimized to allow for ease of synthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 254
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 254
    Illegal NotI site found at 46
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 254
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 254
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 407


[edit]
Categories
Parameters
None